pcat®-control vector (Promega)
90
Structured Review
Promega
pcat®-control vector
Pcat® Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat-control+vector/pcat+basic+plasmid/pmc03820564-152-13-17
Average 90 stars, based on 1 article reviews
Pcat® Control Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcat-control+vector/pcat+basic+plasmid/pmc03820564-152-13-17
Average 90 stars, based on 1 article reviews
pcat®-control vector - by Bioz Stars,
2026-09
90/100 stars
Images
Related Articles
Plasmid Preparation:Article Title: Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. Article Snippet: The 59-untranslated sequence of the cdc2 promoter up to nucleotide 2383 relative to the transcription start site was linked to the CAT gene in Article Title: Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes. Article Snippet: The structure of all constructs was veri®ed by direct DNA sequencing. Article Title: Derepression of HMGA2 Gene Expression in Retinoblastoma Is Associated with Cell Proliferation Article Snippet: In each transfection (as described in 44), 3 106 WERI-Rb1 cells were electroporated with 10 μg of Article Title: Characterization of Ets-binding sequence of human transglutaminase 3 gene promoter. Article Snippet: The Etsand Sp1-transcription factors have been implicated in the epithelial specific expression of transglutaminase 3 gene (TGM3) in vitro.. By electrophoretic mobility shift assay (EMSA), the core motif of Ets-binding sequence of TGM3 was determined as ACAGGAAT ( 118 to 111 bp from transcriptional start site).. However, a sequence extending from 120 to þ10 of TGM3 proximal promoter region failed to induce the expression of CAT reporter in transfected normal human epidermal keratinocytes (NHEKs). Article Title: βig-h3 Induces Keratinocyte Differentiation via Modulation of Involucrin and Transglutaminase Expression through the Integrin α3β1 and the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Article Snippet: A Article Title: Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein. Article Snippet: CACorresponding Author RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symbol APBA3) which interacts with Alzheimer's â-amyloid precursor protein (APP) and has three protein±protein interaction domains, a phosphotyrosine interacting domain (PID) and two PDZ.. Here, we report the genomic structure and mapping of the APBA3.. The gene spans about 11 kb and is composed of ten coding exons and one untranslated exon. Article Title: Regulation of murine myelin proteolipid protein gene expression Article Snippet: To identify putative sequences that direct cell typespecific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice.. These constructs were transfected into murine oligodendrocyte cell lines that transcribe the PLP gene and, hence, should contain the requisite trans-acting factors necessary for PLP gene expression.. Mouse NIH/3T3 fibroblasts were used as a nonglial model. We have finely mapped the PLP promoter region for transcriptional regulatory elements and demonstrate both positive and negative elements, none of which appear to extinguish expression in nonglial cells. Transfection:Article Title: Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. Article Snippet: The 59-untranslated sequence of the cdc2 promoter up to nucleotide 2383 relative to the transcription start site was linked to the CAT gene in Article Title: Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes. Article Snippet: The structure of all constructs was veri®ed by direct DNA sequencing. Article Title: Derepression of HMGA2 Gene Expression in Retinoblastoma Is Associated with Cell Proliferation Article Snippet: In each transfection (as described in 44), 3 106 WERI-Rb1 cells were electroporated with 10 μg of Article Title: Characterization of Ets-binding sequence of human transglutaminase 3 gene promoter. Article Snippet: The Etsand Sp1-transcription factors have been implicated in the epithelial specific expression of transglutaminase 3 gene (TGM3) in vitro.. By electrophoretic mobility shift assay (EMSA), the core motif of Ets-binding sequence of TGM3 was determined as ACAGGAAT ( 118 to 111 bp from transcriptional start site).. However, a sequence extending from 120 to þ10 of TGM3 proximal promoter region failed to induce the expression of CAT reporter in transfected normal human epidermal keratinocytes (NHEKs). Article Title: βig-h3 Induces Keratinocyte Differentiation via Modulation of Involucrin and Transglutaminase Expression through the Integrin α3β1 and the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Article Snippet: A Article Title: Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein. Article Snippet: CACorresponding Author RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symbol APBA3) which interacts with Alzheimer's â-amyloid precursor protein (APP) and has three protein±protein interaction domains, a phosphotyrosine interacting domain (PID) and two PDZ.. Here, we report the genomic structure and mapping of the APBA3.. The gene spans about 11 kb and is composed of ten coding exons and one untranslated exon. Article Title: Regulation of murine myelin proteolipid protein gene expression Article Snippet: To identify putative sequences that direct cell typespecific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice.. These constructs were transfected into murine oligodendrocyte cell lines that transcribe the PLP gene and, hence, should contain the requisite trans-acting factors necessary for PLP gene expression.. Mouse NIH/3T3 fibroblasts were used as a nonglial model. We have finely mapped the PLP promoter region for transcriptional regulatory elements and demonstrate both positive and negative elements, none of which appear to extinguish expression in nonglial cells. Construct:Article Title: Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. Article Snippet: The 59-untranslated sequence of the cdc2 promoter up to nucleotide 2383 relative to the transcription start site was linked to the CAT gene in Article Title: Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes. Article Snippet: The structure of all constructs was veri®ed by direct DNA sequencing. Article Title: Derepression of HMGA2 Gene Expression in Retinoblastoma Is Associated with Cell Proliferation Article Snippet: In each transfection (as described in 44), 3 106 WERI-Rb1 cells were electroporated with 10 μg of Article Title: Characterization of Ets-binding sequence of human transglutaminase 3 gene promoter. Article Snippet: The Etsand Sp1-transcription factors have been implicated in the epithelial specific expression of transglutaminase 3 gene (TGM3) in vitro.. By electrophoretic mobility shift assay (EMSA), the core motif of Ets-binding sequence of TGM3 was determined as ACAGGAAT ( 118 to 111 bp from transcriptional start site).. However, a sequence extending from 120 to þ10 of TGM3 proximal promoter region failed to induce the expression of CAT reporter in transfected normal human epidermal keratinocytes (NHEKs). Article Title: βig-h3 Induces Keratinocyte Differentiation via Modulation of Involucrin and Transglutaminase Expression through the Integrin α3β1 and the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Article Snippet: A Article Title: Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein. Article Snippet: CACorresponding Author RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symbol APBA3) which interacts with Alzheimer's â-amyloid precursor protein (APP) and has three protein±protein interaction domains, a phosphotyrosine interacting domain (PID) and two PDZ.. Here, we report the genomic structure and mapping of the APBA3.. The gene spans about 11 kb and is composed of ten coding exons and one untranslated exon. Article Title: Regulation of murine myelin proteolipid protein gene expression Article Snippet: To identify putative sequences that direct cell typespecific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice.. These constructs were transfected into murine oligodendrocyte cell lines that transcribe the PLP gene and, hence, should contain the requisite trans-acting factors necessary for PLP gene expression.. Mouse NIH/3T3 fibroblasts were used as a nonglial model. We have finely mapped the PLP promoter region for transcriptional regulatory elements and demonstrate both positive and negative elements, none of which appear to extinguish expression in nonglial cells. Expressing:Article Title: Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. Article Snippet: The 59-untranslated sequence of the cdc2 promoter up to nucleotide 2383 relative to the transcription start site was linked to the CAT gene in Article Title: Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes. Article Snippet: The structure of all constructs was veri®ed by direct DNA sequencing. Article Title: Derepression of HMGA2 Gene Expression in Retinoblastoma Is Associated with Cell Proliferation Article Snippet: In each transfection (as described in 44), 3 106 WERI-Rb1 cells were electroporated with 10 μg of Article Title: Characterization of Ets-binding sequence of human transglutaminase 3 gene promoter. Article Snippet: The Etsand Sp1-transcription factors have been implicated in the epithelial specific expression of transglutaminase 3 gene (TGM3) in vitro.. By electrophoretic mobility shift assay (EMSA), the core motif of Ets-binding sequence of TGM3 was determined as ACAGGAAT ( 118 to 111 bp from transcriptional start site).. However, a sequence extending from 120 to þ10 of TGM3 proximal promoter region failed to induce the expression of CAT reporter in transfected normal human epidermal keratinocytes (NHEKs). Article Title: βig-h3 Induces Keratinocyte Differentiation via Modulation of Involucrin and Transglutaminase Expression through the Integrin α3β1 and the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Article Snippet: A Article Title: Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein. Article Snippet: CACorresponding Author RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symbol APBA3) which interacts with Alzheimer's â-amyloid precursor protein (APP) and has three protein±protein interaction domains, a phosphotyrosine interacting domain (PID) and two PDZ.. Here, we report the genomic structure and mapping of the APBA3.. The gene spans about 11 kb and is composed of ten coding exons and one untranslated exon. Article Title: Regulation of murine myelin proteolipid protein gene expression Article Snippet: To identify putative sequences that direct cell typespecific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice.. These constructs were transfected into murine oligodendrocyte cell lines that transcribe the PLP gene and, hence, should contain the requisite trans-acting factors necessary for PLP gene expression.. Mouse NIH/3T3 fibroblasts were used as a nonglial model. We have finely mapped the PLP promoter region for transcriptional regulatory elements and demonstrate both positive and negative elements, none of which appear to extinguish expression in nonglial cells. Positive Control:Article Title: Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. Article Snippet: The 59-untranslated sequence of the cdc2 promoter up to nucleotide 2383 relative to the transcription start site was linked to the CAT gene in Article Title: Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes. Article Snippet: The structure of all constructs was veri®ed by direct DNA sequencing. Article Title: Derepression of HMGA2 Gene Expression in Retinoblastoma Is Associated with Cell Proliferation Article Snippet: In each transfection (as described in 44), 3 106 WERI-Rb1 cells were electroporated with 10 μg of Article Title: Characterization of Ets-binding sequence of human transglutaminase 3 gene promoter. Article Snippet: The Etsand Sp1-transcription factors have been implicated in the epithelial specific expression of transglutaminase 3 gene (TGM3) in vitro.. By electrophoretic mobility shift assay (EMSA), the core motif of Ets-binding sequence of TGM3 was determined as ACAGGAAT ( 118 to 111 bp from transcriptional start site).. However, a sequence extending from 120 to þ10 of TGM3 proximal promoter region failed to induce the expression of CAT reporter in transfected normal human epidermal keratinocytes (NHEKs). Article Title: βig-h3 Induces Keratinocyte Differentiation via Modulation of Involucrin and Transglutaminase Expression through the Integrin α3β1 and the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Article Snippet: A Article Title: Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein. Article Snippet: CACorresponding Author RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symbol APBA3) which interacts with Alzheimer's â-amyloid precursor protein (APP) and has three protein±protein interaction domains, a phosphotyrosine interacting domain (PID) and two PDZ.. Here, we report the genomic structure and mapping of the APBA3.. The gene spans about 11 kb and is composed of ten coding exons and one untranslated exon. Article Title: Regulation of murine myelin proteolipid protein gene expression Article Snippet: To identify putative sequences that direct cell typespecific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice.. These constructs were transfected into murine oligodendrocyte cell lines that transcribe the PLP gene and, hence, should contain the requisite trans-acting factors necessary for PLP gene expression.. Mouse NIH/3T3 fibroblasts were used as a nonglial model. We have finely mapped the PLP promoter region for transcriptional regulatory elements and demonstrate both positive and negative elements, none of which appear to extinguish expression in nonglial cells. Cell Culture:Article Title: Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. Article Snippet: The 59-untranslated sequence of the cdc2 promoter up to nucleotide 2383 relative to the transcription start site was linked to the CAT gene in Article Title: Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes. Article Snippet: The structure of all constructs was veri®ed by direct DNA sequencing. Article Title: Derepression of HMGA2 Gene Expression in Retinoblastoma Is Associated with Cell Proliferation Article Snippet: In each transfection (as described in 44), 3 106 WERI-Rb1 cells were electroporated with 10 μg of Article Title: Characterization of Ets-binding sequence of human transglutaminase 3 gene promoter. Article Snippet: The Etsand Sp1-transcription factors have been implicated in the epithelial specific expression of transglutaminase 3 gene (TGM3) in vitro.. By electrophoretic mobility shift assay (EMSA), the core motif of Ets-binding sequence of TGM3 was determined as ACAGGAAT ( 118 to 111 bp from transcriptional start site).. However, a sequence extending from 120 to þ10 of TGM3 proximal promoter region failed to induce the expression of CAT reporter in transfected normal human epidermal keratinocytes (NHEKs). Article Title: βig-h3 Induces Keratinocyte Differentiation via Modulation of Involucrin and Transglutaminase Expression through the Integrin α3β1 and the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Article Snippet: A Article Title: Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein. Article Snippet: CACorresponding Author RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symbol APBA3) which interacts with Alzheimer's â-amyloid precursor protein (APP) and has three protein±protein interaction domains, a phosphotyrosine interacting domain (PID) and two PDZ.. Here, we report the genomic structure and mapping of the APBA3.. The gene spans about 11 kb and is composed of ten coding exons and one untranslated exon. Article Title: Regulation of murine myelin proteolipid protein gene expression Article Snippet: To identify putative sequences that direct cell typespecific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice.. These constructs were transfected into murine oligodendrocyte cell lines that transcribe the PLP gene and, hence, should contain the requisite trans-acting factors necessary for PLP gene expression.. Mouse NIH/3T3 fibroblasts were used as a nonglial model. We have finely mapped the PLP promoter region for transcriptional regulatory elements and demonstrate both positive and negative elements, none of which appear to extinguish expression in nonglial cells. Sequencing:Article Title: Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. Article Snippet: The 59-untranslated sequence of the cdc2 promoter up to nucleotide 2383 relative to the transcription start site was linked to the CAT gene in Article Title: Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes. Article Snippet: The structure of all constructs was veri®ed by direct DNA sequencing. Article Title: Derepression of HMGA2 Gene Expression in Retinoblastoma Is Associated with Cell Proliferation Article Snippet: In each transfection (as described in 44), 3 106 WERI-Rb1 cells were electroporated with 10 μg of Article Title: Characterization of Ets-binding sequence of human transglutaminase 3 gene promoter. Article Snippet: The Etsand Sp1-transcription factors have been implicated in the epithelial specific expression of transglutaminase 3 gene (TGM3) in vitro.. By electrophoretic mobility shift assay (EMSA), the core motif of Ets-binding sequence of TGM3 was determined as ACAGGAAT ( 118 to 111 bp from transcriptional start site).. However, a sequence extending from 120 to þ10 of TGM3 proximal promoter region failed to induce the expression of CAT reporter in transfected normal human epidermal keratinocytes (NHEKs). Article Title: βig-h3 Induces Keratinocyte Differentiation via Modulation of Involucrin and Transglutaminase Expression through the Integrin α3β1 and the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Article Snippet: A Article Title: Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein. Article Snippet: CACorresponding Author RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symbol APBA3) which interacts with Alzheimer's â-amyloid precursor protein (APP) and has three protein±protein interaction domains, a phosphotyrosine interacting domain (PID) and two PDZ.. Here, we report the genomic structure and mapping of the APBA3.. The gene spans about 11 kb and is composed of ten coding exons and one untranslated exon. Article Title: Regulation of murine myelin proteolipid protein gene expression Article Snippet: To identify putative sequences that direct cell typespecific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice.. These constructs were transfected into murine oligodendrocyte cell lines that transcribe the PLP gene and, hence, should contain the requisite trans-acting factors necessary for PLP gene expression.. Mouse NIH/3T3 fibroblasts were used as a nonglial model. We have finely mapped the PLP promoter region for transcriptional regulatory elements and demonstrate both positive and negative elements, none of which appear to extinguish expression in nonglial cells. DNA Sequencing:Article Title: Modulation of E2F activity is linked to interferon-induced growth suppression of hematopoietic cells. Article Snippet: The 59-untranslated sequence of the cdc2 promoter up to nucleotide 2383 relative to the transcription start site was linked to the CAT gene in Article Title: Transcriptional repression of the E2F-1 gene by interferon-alpha is mediated through induction of E2F-4/pRB and E2F-4/p130 complexes. Article Snippet: The structure of all constructs was veri®ed by direct DNA sequencing. Article Title: Derepression of HMGA2 Gene Expression in Retinoblastoma Is Associated with Cell Proliferation Article Snippet: In each transfection (as described in 44), 3 106 WERI-Rb1 cells were electroporated with 10 μg of Article Title: Characterization of Ets-binding sequence of human transglutaminase 3 gene promoter. Article Snippet: The Etsand Sp1-transcription factors have been implicated in the epithelial specific expression of transglutaminase 3 gene (TGM3) in vitro.. By electrophoretic mobility shift assay (EMSA), the core motif of Ets-binding sequence of TGM3 was determined as ACAGGAAT ( 118 to 111 bp from transcriptional start site).. However, a sequence extending from 120 to þ10 of TGM3 proximal promoter region failed to induce the expression of CAT reporter in transfected normal human epidermal keratinocytes (NHEKs). Article Title: βig-h3 Induces Keratinocyte Differentiation via Modulation of Involucrin and Transglutaminase Expression through the Integrin α3β1 and the Phosphatidylinositol 3-Kinase/Akt Signaling Pathway Article Snippet: A Article Title: Genomic organization of the human X11L2 gene (APBA3), a third member of the X11 protein family interacting with Alzheimer's beta-amyloid precursor protein. Article Snippet: CACorresponding Author RECENTLY we cloned a new member of the X11 protein family, X11L2 (gene symbol APBA3) which interacts with Alzheimer's â-amyloid precursor protein (APP) and has three protein±protein interaction domains, a phosphotyrosine interacting domain (PID) and two PDZ.. Here, we report the genomic structure and mapping of the APBA3.. The gene spans about 11 kb and is composed of ten coding exons and one untranslated exon. Article Title: Regulation of murine myelin proteolipid protein gene expression Article Snippet: To identify putative sequences that direct cell typespecific expression and/or enhance proteolipid protein (PLP) gene expression, glial or nonglial cells were transfected with various PLP-luciferase constructs that collectively span the entire mouse PLP-specific DNA present in a transgene known to direct cell type specificity in transgenic mice.. These constructs were transfected into murine oligodendrocyte cell lines that transcribe the PLP gene and, hence, should contain the requisite trans-acting factors necessary for PLP gene expression.. Mouse NIH/3T3 fibroblasts were used as a nonglial model. We have finely mapped the PLP promoter region for transcriptional regulatory elements and demonstrate both positive and negative elements, none of which appear to extinguish expression in nonglial cells. |